| | |
|---|
| Cell line: | SW-1710 |
| DSMZ no.: | ACC 426 |
| Species: | human (Homo sapiens) |
| Cell type: | urinary bladder transitional cell cacrinoma |
| Origin: | established from the bladder tumor of an 84-year-old woman following transurethral tumor resection in 1977 |
| Reference(s): | 14700 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like, elongated cells growing adherently as monolayers; image; image |
| Medium: | 85% DMEM + 15% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:3 to 1:5 every 2-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 25-32 hours |
| Harvest: | cell harvest of ca. 6-8 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypotetraploid karyotype with 8% polyploidy - 77-84<4n>X, -X, -X, -X, -4, -4, -5, -5, +6, +6, -8, -9, -10, -11, -13, -14, -14, -16, -19, +20, +20, +4mar, der(X)t(X;6)(q11-13;q25-q27), del(2)(p12)x2, add(3)(p14-21), der(6)t(X;6)(q11-13;q25-27)x2, der(6)del(6)(p11p12.2)add(6)(q25-27)x2, add(13)(q33-34) - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
