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Cell line: | DU-4475 |
DSMZ no.: | ACC 427 |
Species: | human (Homo sapiens) |
Cell type: | breast carcinoma |
Origin: | established from the local metastatic cutaneous nodule of an undifferentiated carcinoma of a 62-year-old woman which developed seven years after radical mastectomy of a poorly differentiated ductal carcinoma of the breast; triple-negative; cells were described in the literature to grow readily in athymic nude mice and to produce tumors from which cells could be introduced into culture in vitro; cells reportedly express components of milk fat globules; RNA sequence data are available (see Ref 40906 and RNA-Seq) |
Reference(s): | 14529, 14704, 18232, 18234, 40906 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | large round cells growing singly or in clusters in suspension; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.2 x 106 cells/ml; split saturated culture 1:2 to 1:3 once a week;
maximum cell density at ca. 0.5 x 106/ml; due to cluster formation, cells are difficult to count; cultures may contain large amounts of debris and viability may be consistently below 70% |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 80-100 hours |
Harvest: | cell harvest of ca. 0.3-0.4 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded near-tetraploid karyotype with 12% polyploidy - 88-93<4n>XXXX, +1, +1, -5, -6, +9, -10, -10, +15, +15, -16, -16, +22, +4mar, i(1q)x2, ?add(1)(p35-36)x2, ?i(5p)x2, add(6)(p11), add(6)(p1?), del(6)(q25), add(9)(q35), del(11)(q24)x2, add(15)(p11)x2, add(17)(p1?)x2, del(21)(q22.2)x2 - sideline with -20, -20, +del(7)(p11) - gain of 1q and loss of 6q typical in breast carcinoma - resembles published karyotype |
Molec. Genetics: | triple-negative confirmed by absence of ER, PR and HER2 in Western blots |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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