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| Cell line: | 42-MG-BA |
| DSMZ no.: | ACC 431 |
| Species: | human (Homo sapiens) |
| Cell type: | glioblastoma |
| Origin: | established from the temporal lobe brain biopsy from a 63-year-old man with glioblastoma multiforme in 1990 (prior to treatment) |
| Reference(s): | 14846 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes; image; image; image |
| Medium: | 90% mixture of RPMI 1640 + MEM (with Earle's salts) (at 1:1) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-6 days using trypsin/EDTA; saturation density at ca. 0.6 x 105/cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | cell harvest of maximal 0.3-0.5 x 105/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertetraploid karyotype with 8% polyploidy - 88-95<4n>XX, -Y, -Y, +4, -5, -5, -7, -8, -9, -9, +11, +11, -13, -13, +14, +14, +15, -16, -17, -17, -18, -18, -19, -21, -22, +14mar, add(1)(p1?), del(1)(q21.2), del(2)(q13)x2, dup(2)(p12p2?4)add(2)(p2?4), del(3)(q13.3), add(4)(p11), add(5)(p1?5), add(6)(q2?)x2, add(7)(q11)x2, add(7)(q3?), del(9)(p21p22)x2, del(11)(p11)x2, inv(12)(q12q21)x2, der(12)del(p11)t(7;12)(q21;q1?4)x2, add(13)(p11)x2, add(14)(q32)x2, add(21)(p11)x2 |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
