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Cell line: | EVSA-T |
DSMZ no.: | ACC 433 |
Species: | human (Homo sapiens) |
Cell type: | breast carcinoma |
Origin: | established from the malignant ascitic effusion from a woman with metastatic breast carcinoma; described originally as being estrogen receptor-negative and progesterone receptor-positive (the latter not confirmed by DSMZ);
RNA sequence data are available (see Ref 40906 and RNA-Seq) |
Reference(s): | 14400, 14718, 14447, 14702, 15191, 40906 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epitheloid cells growing in monolayers; image; image; image |
Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine |
Subculture: | seed out at ca. 2-5 x 106 cells/80 cm2; split semi-confluent culture 1:3 every 3-4 days using trypsin/EDTA, dissociate cells with a pipette (10 ml) during trypsination; after thawing and in the first week, viability and adherence may be low (about 50%) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of about 20-30 x 106 per 80cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotriploid karyotype with 4% polyploidy - 62(55-64)<3n>XXX, +1, -2, -5, -5, +6, -8, -9, -10, -13, -13, -14, -15, -16, -17, -18, -20, -22, +6-8mar, add(X)(p11), der(X)t(X;9)(q21;q11), add(1)(p1?1), der(1)del(1)(p36)dup(1)(q22q32), del(1)(q11), der(1;4)(q10;q10)del(4)(q1?13q?23), der(2)t(2;5)(p11;q13)add(2)(q3?5), der(3)t(3;5)(p14;q11), del(4)(q22), del(6)(q25q27), del(6)(p11), add(6)(p23), del(7)(p14), add(8)(q24), ider(8)(q10)ins(8;?)dup(8)(?q21q24)add(8)(q24), add(11)(p15), add(12)(p13), del(12)(p13), dup(13)(q21q33), add(21)(p11), add(21)(p11), large submetacentric der(8) hsr marker present |
Molec. Genetics: | Western blot : ER, PR, AR negative while positive for HER2 |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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