| |
---|
Cell line: | HSB-2 |
DSMZ no.: | ACC 435 |
Species: | human (Homo sapiens) |
Cell type: | T cell leukemia |
Origin: | established in 1966 from the peripheral blood of an 11-year-old boy with lymphosarcoma that had progressed to acute lymphoblastic leukemia (ALL); cell line was established and initially passaged in newborn hamsters; cells were described as carrying the t(1;7)(p34;q34) involving the LCK and TRB (LCK-TCRB) genes and a submicroscopic del(1)(p32) leading to the STIL-TAL1 (SIL-SCL) fusion gene; one of the oldest immature T cell lines, also known in the literature as CCRF-HSB-2 |
Reference(s): | 14345, 14346 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round to polygonal cells, growing singly or in dense clumps in suspension; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.3-0.4 x 106 cells/ml; maintain at ca. 0.4-1.0 x 106 cells/ml; split saturated culture 1:3 every 2-3 days; maximal density at ca. 1.3 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 25 hours |
Harvest: | cell harvest of ca. 0.8 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
Immunology: | CD2 -, cyCD3 +, smCD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, TCRalpha/beta -, TCRgamma/delta -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human pseudodiploid karyotype with 4% polyploidy - 46(42-46)<2n>XY, t(1;7)(p34;q35), carries t(1;7) effecting LCK-TRB (LCK-TCRB) fusion, resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |