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| Cell line: | MOLT-13 (sister to MOLT-14) |
| DSMZ no.: | ACC 436 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia |
| Origin: | established from the bone marrow of a 2-year-old girl with T-ALL (T cell acute lymphoblastic leukemia, FAB L2) at relapse in 1983; MOLT-14 (ACC 437) is a simultaneous sister cell line derived from the same patient sample with similar or identical features; by STR analysis, MOLT-13 and MOLT-14 are genetically identical |
| Reference(s): | 14477 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, mostly round cells with processes and different in size, growing in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/ml in 12-well plates; maintain at a minimum of ca. 1 x 106 cells/ml; cells grow slowly, split saturated culture 1:2 every 5-7 days and change medium twice weekly; cells rarely exceed 80% viability |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 80-120 hours |
| Harvest: | maximal density at ca. 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD2 -, CD3 +, CD5 +, CD7 +, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to that of MOLT-14 |
| Cytogenetics: | human pseudodiploid karyotype with 4% polyploidy - 46(44-48)<2n>XX, der(17)t(17;17)(p13;q22.1) - resembles published karyotype - karyotypically identical to MOLT-14 |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
