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| Cell line: | CAL-72 |
| DSMZ no.: | ACC 439 |
| Species: | human (Homo sapiens) |
| Cell type: | osteosarcoma |
| Origin: | established from a chemotherapy-resistant tumor sample obtained from the left knee of a 10-year-old boy with anaplastic osteoblastic sarcoma (Rosen grade IIB) in 1989 |
| Reference(s): | 14896 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblastoid cells growing adherently in monolayers; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2;
split confluent culture 1:3 to 1:10 every 4-6 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5-10% CO2 |
| Doubling time: | ca. 40-50 hours |
| Harvest: | cell harvest of ca. 7 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human, bimodal hyperdiploid/hypertetraploid karyotype with 12% polyploidy - 45-56<2n>X, -Y, +1, +1, -2, -2, +8, +11, -13, -15, +19, -20, -20, -22, -22, +15-20mar, +dmin, add(X)(q21), der(1;19)(q10;q10), add(1)(p3?5)add(1)(q3?), add(3)(q2?9)x2, add(6)(q11), del(7)(q11), add(8)(q24), add(8)(q11), add(11)(p1?), del(11)(q13), add(14)(q3?)x2, add(16)(p11), add(16)(q23), add(19)(p11), add(21)(p11) (hyperdiploid clone) - additional rearrangements, including der(1;8)(q10;q10) and idic(8;?)(p10;?) present in hypertetraploid clone |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
