| | |
|---|
| Cell line: | ELM-I-1 |
| DSMZ no.: | ACC 44 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | erythroleukemia |
| Origin: | established from an X-irradiated C3H/HeNSIc mouse; cells were described in the literature to be stroma-independent, responsive to erythropoietin and to produce hemoglobin (augmented by IL-3) |
| Reference(s): | 14958, 14632 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | large, round, single cells in suspension; image; image |
| Medium: | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS |
| Subculture: | maintain at 0.1-0.5 x 106 cells/ml; split saturated culture 1:3; maximal density at about 2-5 x 106 cells/ml; seed out at ca. 0.2-0.4 x 106 cells/ml; note that after thawing cell viability may be as low as 50%, but cells will recover quickly within a week or so |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | 20-40 h |
| Harvest: | saturation density of about 0.5-1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
