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| Cell line: | MS-5 |
| DSMZ no.: | ACC 441 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | stromal cells |
| Origin: | established by irradiation of the adherent cells in long-term bone marrow cultures derived from C3H/HeNSlc strain mice |
| Reference(s): | 14633 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblastic cells growing in monolayers; image; image; image; image; image |
| Medium: | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% horse serum (or h.i. FBS) + 2 mM L-glutamine + 2 mM sodium pyruvate |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every third day using trypsin/EDTA; maximal density at ca. 15 x 106 cells/175 cm2; CAVEAT: cells may detach spontaneously in aggregates which are difficult to disperse |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertriploid karyotype |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
