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| Cell line: | AC-1M32 |
| DSMZ no.: | ACC 442 |
| Species: | human (Homo sapiens) |
| Cell type: | choriocarcinoma-trophoblast hybrid |
| Origin: | one of five phenotypically and cytogenetically distinct subclones of a hybrid (ACH1P, DSM ACC 481) formed by fusing an HGPRT-defective mutant (AC1-1) of JEG-3 human choriocarcinoma (DSM ACC 463) with primary human trophoblast (chorion laeve) cells |
| Reference(s): | 14543, 15146 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently in monolayers; image; image; image |
| Medium: | 90% Ham's F12 + 10% h.i. FBS |
| Subculture: | split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA; seed out at ca. 0.1-0.2 x 105 cells/cm2; maintain at ca. 0.1-1.3 x 105 cells/cm2; reaches max. density of ca. 1.8 x 105 cells/cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-30 hours |
| Harvest: | cell harvest of ca. 1.0-1.5 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | near identical DNA profiles shared by the AC-sister cell lines, partly identical with JEG-3 and BEWO |
| Cytogenetics: | human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-104<4n>XXXY, +1, +2, +3, -4, +7, +9, -13, -13, -15, +16, +16, -18, -20, +21, +22, +5-7mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), del(1)(p11), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32), add(9)(p24), add(11)(p15), i(13q), add(15)(p13), add(19)(p13) - sideline with: idem, +7, +16, -18, del(1)(p11), +del(1)(p22) - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
