| |
---|
Cell line: | FU-OV-1 |
DSMZ no.: | ACC 444 |
Species: | human (Homo sapiens) |
Cell type: | ovarian carcinoma |
Origin: | established post-hysterectomy from the tumor tissue of a 65-year-old woman with malignant ovarian carcinoma (stage: FIGO IIIc; histology: poorly differentiated serous papillary adenocarcinoma) in 1989 |
Reference(s): | 14510 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like adherent cells growing in monolayers; image; image; image; image |
Medium: | 80-90% mixture of Ham's F12 + DMEM (at 1:1) + 10-20% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 120 hours |
Harvest: | cell harvest of ca. 5-10 x 106 cells/175 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypotriploid karyotype with 12% polyploidy - 58-62<3n>X, -X, -X, -1, -2, -3, -4, -7, +9, -10, -12, -13, -14, -16, -17, -17, -18, -19, -20, -20, -22, +fra, +dm, del(2)(p23), inv(3)(p25q29), del(4)(q21/22), add(5)(p11), add(6)(q27), add(9)(p11), add(11)(p15)x2, add(11)(q24), add(14)(p11), add(14)(p11), add(16)(p13), add(19)(q13)x2, add(?21)(p11)x2 - resembles published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |