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| Cell line: | FU-OV-1 |
| DSMZ no.: | ACC 444 |
| Species: | human (Homo sapiens) |
| Cell type: | ovarian carcinoma |
| Origin: | established post-hysterectomy from the tumor tissue of a 65-year-old woman with malignant ovarian carcinoma (stage: FIGO IIIc; histology: poorly differentiated serous papillary adenocarcinoma) in 1989 |
| Reference(s): | 14510 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like adherent cells growing in monolayers; image; image; image; image; image |
| Medium: | 80-90% mixture of Ham's F12 + DMEM (at 1:1) + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 120 hours |
| Harvest: | cell harvest of ca. 5-10 x 106 cells/175 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypotriploid karyotype with 12% polyploidy - 58-62<3n>X, -X, -X, -1, -2, -3, -4, -7, +9, -10, -12, -13, -14, -16, -17, -17, -18, -19, -20, -20, -22, +fra, +dm, del(2)(p23), inv(3)(p25q29), del(4)(q21/22), add(5)(p11), add(6)(q27), add(9)(p11), add(11)(p15)x2, add(11)(q24), add(14)(p11), add(14)(p11), add(16)(p13), add(19)(q13)x2, add(?21)(p11)x2 - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
