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Cell line: | CAL-33 |
DSMZ no.: | ACC 447 |
Species: | human (Homo sapiens) |
Cell type: | tongue squamous cell carcinoma |
Origin: | established from the surgically removed fragment of a tongue lesion from a 69-year-old man with moderately differentiated squamous cell carcinoma of the tongue in 1983 (prior to therapy); described to be tumorigenic in nude mice; listed in the CCLE (ref 18232) with genomic and protein analyses |
Reference(s): | 14562, 18232, 40405 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing adherent in monolayers; image; image; image; image |
Medium: | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine |
Subculture: | seed out at 2-4 x 106 cells/175 cm2; split confluent culture 1:3 to 1:5 every 4-5 days using trypsin/EDTA (for about 10 min) |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 40 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy 49(45-49)<2n>XY/XYY, +7, +9, -18, +20, +mar, add(X)(p22), add(3)(p22-24), i(7p), i(8q), add(9)(p24), del(9)(p2?2) - resembles published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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