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| Cell line: | BC-3C |
| DSMZ no.: | ACC 450 |
| Species: | human (Homo sapiens) |
| Cell type: | urinary bladder transitional cell carcinoma |
| Origin: | derived from the invasive solid transitional cell carcinoma of the bladder of a 82-year-old woman (grade IV, stage C); cell growth described as being inhibited by EGF |
| Reference(s): | 14857 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid adherent cells growing adherently in monolayers and in multilayer foci; image; image; image; image |
| Medium: | 90% McCoy's 5A + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-3 x 106 cells in a T75 flask; recover and initially culture cells in 20% FBS; split confluent culture up to 1:10 every 3-7 days using trypsin/EDTA; maximum density of ca. 3.5 x 105 cells/cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 25-30 hours |
| Harvest: | cell harvest of ca. 2-3 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 10% polyploidy - 65-72<3n>XX, -X, -2, -4, +9, +9, -11, -13, +14, +16, -17, -17, -18, +19, +20, +20, +2-3mar, add(2)(p1?), del(3)(q2?2), der(5;7)(q10;q10), del(9)(p2?2p2?3), add(12)(q24) - sideline with del(2)(q2?), i(3q), add(7)(p22), add(8)(p12), add(11)(q22-24), i(?17p), i(17q) and extra numerical changes - matches published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
