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| Cell line: | RO |
| DSMZ no.: | ACC 452 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells (from patient with severe combined immunodeficiency, SCID) |
| Origin: | established from the peripheral blood of a 3-year-old boy with severe combined immunodeficiency (SCID) by EBV-transformation using the cell line B95-8 as source for EBV; cells were described as lacking MHC class II molecules |
| Reference(s): | 14453, 14983, 15172, 15173 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing in suspension, singly or in small clusters; image; image; |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | split saturated culture at 1:2 every two days; seed out at ca. 1.0 x 106 cells/ml; it is advisable to culture the cells initially in 24-well-plates; cell growth may be slow for the first week maximal density at ca. 2.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-50 hours |
| Harvest: | cell harvest of ca. 1.0-1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR -, cyIgG -, cyIgM +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 8% polyploidy - 46(43-46)<2n>XY, no consistent abnormality present |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
