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| Cell line: | JAR |
| DSMZ no.: | ACC 462 |
| Species: | human (Homo sapiens) |
| Cell type: | choriocarcinoma |
| Origin: | established from the trophoblastic tumor of the placenta of a 24-year-old woman, but the cell line is from the male fetus |
| Reference(s): | 15154,18252 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing adherent in monolayers and in clusters; image; image; image |
| Medium: | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture: | split culture 1:5 to 1:10 every 3-4 days before reaching confluency using trypsin/EDTA; seed out at ca. 3-4 x 106 cells/80 cm2; confluent cultures tend to form aggregates, that loosely adhere to the culture vessel surface; however, these aggregates are difficult to disperse and may need trypsin treatment after a centrifugation and washing step |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 20-30 hours |
| Harvest: | cell harvest of ca. 10-15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded near-triploid karyotype with 27% polyploidy - 60-70<3n>XXY, +1, -2, -5, -7, -8, -9, -13, -13, -14, -15, -17, -19, -21,+12mar, +acf, add(X)(p22), del(1)(p3?p3?), add(1)(q11), der(1)add(1)(p11)add(1)(q4?), inv(3)(p25.2q24), add(3)(q13), add(4)(q?31), del(4)(q2?2), add(9)(p21), add(12)(q24), i(13)(q10)dup(13)(q11q34), add(16)(q24), add(17)(p12), add(18)(p11.2) - sideline with add(2)(p2?), del(8)(q22), add(21)(p11) - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
