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Cell line: | JEG-3 |
DSMZ no.: | ACC 463 |
Species: | human (Homo sapiens) |
Cell type: | choriocarcinoma |
Origin: | established from a human gestational choriocarcinoma which was taken at autopsy from a cerebral metastasis; cells were initially propagated in hamster cheek pouches by serial passage prior to cultivation in culture; serial sister cell line of cell line BEWO (DSMZ ACC 458) |
Reference(s): | 14682, 14836, 14543, 18252, 18253 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epithelial-like cells growing as monolayer and carrying many intracellular dark granula; image;image |
Medium: | 90% Ham's F12 + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2; maintain at ca. 10 x 106 cells/175 cm2; split confluent culture up to 1:10 every 3-4 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 24 hours |
Harvest: | cell harvest of ca. 25 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype with 12% polyploidy - 71(69-72)<3n>XXY, +2, +5, +9, -11, -13, -18, +4-6mar, ?del(X)(p11.4), der(4)t(4;11)(p16;q13), add(6)(q2?7)x1-2, add(7)(q32), add(7)(p22), i(13q), add(15)(p11), add(19)(p13) - sideline with add(8)(p1?), add(10)(q21), ?del(18)(q22) - resembles published karyotype and (marginally) that of BEWO |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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