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| Cell line: | HPB-ALL |
| DSMZ no.: | ACC 483 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia |
| Origin: | established from the peripheral blood of a 14-year-old boy with ALL and thymoma at diagnosis in 1973. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s): | 14785, 15217, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorph cells growing singly or in clusters in suspension; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/ml; after thawing, we recommend to use initially 24-well-plates and 20% FBS; cell viability may be as low as 50% after thawing and cells may do not grow during the first week, then recover slowly; in this first week do not dilute, only exchange spent medium; when growing is resumed, split saturated culture 1:2 to 1:3 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | maximum density of about 4 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD2 +, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | pseudodiploid human karyotype with 8% polyploidy - 46<2n>XY, der(1)t(1;16)(q22;p11-12)add(16)(p13), del(2)(p24), del(3)(p11), der(5)t(1;5)(q22-24;q32-33), r(16)(?p12?q12) - sideline with -3 instead of del(3) - resembles published karyotype |
| Molec. Genetics: | expression of TLX3 confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
