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Cell line: | HCC-33 |
DSMZ no.: | ACC 487 |
Species: | human (Homo sapiens) |
Cell type: | small cell lung carcinoma |
Origin: | established from the pleural effusion of a 52-year-old man with small cell lung carcinoma; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available HCC-33BL (DSMZ ACC 889); listed in the CCLE (ref 18232) |
Reference(s): | 14567, 15046, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round to ovoid cells growing singly and mainly in clumps in suspension; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | due to clump formation, cell number and viability is difficult to determine; if possible, seed out at ca. 1.0 x 106 cells/ml or suspend the cells of one vial in a T75 flask;
cell clumps should be only very carefully resuspended, avoid strong shearing; split saturated culture 1:2 to 1:3 every 2-5 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40 hours |
Harvest: | cell harvest of ca. 2 x 106 cells/ml (difficult to determine) |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypotriploid karyotype with 8% polyploidy - 61-68<3n>XX, -Y, +1, +1, +3, -10, -12, -13, +17, -20, -22, +3-5 mar, ider(1)(q10)del(1)(?q12?q43)x2, add(1)(p36), del(1)(p32)x1-2, dup(2)(p23p24), der(3;17)(q10;q10)x1-2, add(8)(p11), del(9)(q21), der(12)t(11;12)(q13.4;q24.3), add(13)p11) - sideline with add(5)(p11), add(15)(p11), add(17)(p1?), del(22)(q12) - variable dmin present in most cells - dup(2) includes MYCN locus |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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