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| Cell line: | RH-30 |
| DSMZ no.: | ACC 489 |
| Species: | human (Homo sapiens) |
| Cell type: | rhabdomyosarcoma |
| Origin: | established from the bone marrow metastasis of a 17-year-old man with undifferentiated (standard classification) or unclassified (Palmer classification) alveolar rhabdomyosarcoma; cells were described as carrying a p53 mutation, expressing high levels of both myogenin and myoD, and expressing the Pax3/FKHR fusion protein secondary to the t(2;13)(q35;q14) translocation |
| Reference(s): | 14467, 14558, 15010 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epithelial-like cells growing as monolayer; image; image; image |
| Medium: | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1 x 106 cells/80 cm2;
split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 35 hours |
| Harvest: | cell harvest of ca. 10-15 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | expression of fusion gene PAX3-FOXO1/FKHR confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
