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Cell line: | HCC-366 |
DSMZ no.: | ACC 492 |
Species: | human (Homo sapiens) |
Cell type: | non-small cell lung carcinoma |
Origin: | established from the tumor of an 80-year-old woman with non-small cell lung carcinoma (subtype adeno-squamous carcinoma); cells were described in the literature to carry fractional allellic loss with loss of heterozygosity at various hot spots; listed in the CCLE (ref 18232) with extended genome and protein analyses; matched EBV+ B-lymphoblastoid cell line (B-LCL, HCC-366BL) is available (DSMZ ACC 895) |
Reference(s): | 14821, 14849, 15046, 14567, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epitheloid cells growing as monolayer; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca.0.5-1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA; maximal density at ca. 2 x 106 cells/80 cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 72-96 hours |
Harvest: | cell harvest of ca. 4 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hyperdiploid karyotype with 12% polyploidy - 48-56<2n>XX, +1, +1, -2, +4, +7, +8, +11, -13, -15, -18, +7-9mar, add(X)(p11), add(1)(p22), add(1)(q11)x1-2, add(4)(q31)x1-2, del(8)(p12), del(8)(q13), add(10)(p13), add(14)(p11), add(17)(p12) |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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