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Cell line: | RH-1 |
DSMZ no.: | ACC 493 |
Species: | human (Homo sapiens) |
Cell type: | Ewing sarcoma |
Origin: | established from the primary tumor of a young boy patient; originally described as an embryonal rhabdomyosarcoma obtained as xenograft. However, according to gene expression profiling and expression of EWS-FLI1 gene fusion transcripts, the cell line is evidently related to Ewing sarcoma; the cell line is also named EW8(Rh1) or EW-8 in literature |
Reference(s): | 14789, 15159, 18251 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions. |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing adherently in monolayers; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | split confluent culture 1:4 to 1:20 every 3-10 days using trypsin/EDTA (short exposure only); seed out at ca. 0.5-1.0 x 106 cells/80 cm2; maximal density at ca. 4 x 105 cells/cm2 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 1-2 x 105 cells/cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotriploid karyotype with 12% polyploidy - 67(61-67)<3n>XX, -Y, +2, -3, +7, -8, -10, +12, -14, -15, -16, +19, -22, add(X)(p22), del(1)(p3?5p3?6), del(2)(p14)x1-2, del(2)(q23), del(9)(p11p13), ?add(11)(q25)x2, der(11)t(2;11)(q?3?q?3?)t(2;3)(q3?;p21), add(12)(q13)/dup(12)(q13q21), del(13)(?q21), der(14)t(Y;14)(q11.2;p11)x2, der(16)t(16;22)(q22;q22)x2, del(18)(q21), add(19)(q13), del(22)(q13) - sideline with +7, +21, add(2)(q11), add(13)(p11) - absence of t(2;13) confirmed by FISH |
Molec. Genetics: | expression of fusion gene EWS-FLI1 confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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