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Cell line: | HCC-15 |
DSMZ no.: | ACC 496 |
Species: | human (Homo sapiens) |
Cell type: | non-small cell lung carcinoma |
Origin: | established from the tumor of a 47-year-old man with non-small cell lung carcinoma (subtype squamous carcinoma); listed in the CCLE (ref 18232) with extended genome and protein analyses; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-15BL, DSMZ ACC 890) |
Reference(s): | 14567, 15046, 14849, 18232, 40404 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epitheloid cells growing as monolayer; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2;
split confluent culture 1:4 to 1:6 every 4-5 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 8 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypodiploid karyotype with 16% polyploidy - 37(34-38)<2n>X, -X, -4, -13, -14, -17, -18, -19, -20, -22, +1-2mar, del(3)(q13), der(5;19)(q10;q10)add(19)(q13), add(9)(p2?), del(10)(p12-13), add(12)(q24.3), del(20)(?p12), add(22)(q1?) - sideline with add(8)(p12), del(7)(q21) |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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