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| Cell line: | MEC-1 |
| DSMZ no.: | ACC 497 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic B cell leukemia |
| Origin: | established in 1993 from the peripheral blood of a 61-year-old man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL); serial sister cell line of MEC-2 (DSMZ ACC 500). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
| Reference(s): | 14980, 40446, 40447, 18187 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorphic cells growing in suspension, singly or partly in small aggregates, a few cells are slightly adherent; image; image |
| Medium: | 90% Iscove's MDM + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; after thawing, during the first week cells should be kept with 20% FBS initially; culture in 24-well-plate is of advantage; maintain at about 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | cell harvest of about 2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MEC-2 (ACC 500) |
| Cytogenetics: | human near-diploid karyotype with 10% polyploidy - 46(44-47)<2n>XY, -2, +7, -12, +1-2mar, t(1;6)(q22-23;p21), add(7)(q11), der(10)(10pter->q22::?::2q11->qter), del(17)(p11) - small acf/mar present in most cells - resembles published karyotype |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
