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| Cell line: | TT2609-C02 |
| DSMZ no.: | ACC 510 |
| Species: | human (Homo sapiens) |
| Cell type: | follicular thyroid carcinoma |
| Origin: | established in 1996 from the tumor tissue of a 57-year-old man with local recurrence of a previously iodine-irradiated follicular thyroid cancer (well-differentiated with dispersed poorly differentiated areas). Cells express the transcriptionally inactive delta p53 isoform. A sister cell line with a truncated delta p53 -/- is also available (ACC 859). Useful for studies with p53 family members p63 and p73 |
| Reference(s): | 14552 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small fibroblastoid cells growing adherently in monolayers; image; image |
| Medium: | 80-85% RPMI 1640 + 15-20% h.i. FBS + 1 mM sodium pyruvate + insulin-transferrin-selenite medium supplement |
| Subculture: | split confluent culture 1:3 to 1:4 every week using trypsin/EDTA (for 10 min); seed out at ca. 1 x 106 cells/25 cm2; maintain at ca. 8 x 106 cells/175 cm2; maximal density at about 14 x 106 cells/175 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-80 hours |
| Harvest: | cell harvest of about 10 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 4% polyploidy - 69-75<3n>XXYY, -2, +8, +8, +9, +10, +14, -15, -16, +17, -22, +1-2mar, der(1)t(1;2)(p32;q11-13), add(4)(p15.2), ?inv(8)(q12q21.2)x1-2, add(9)(p24)x2, i(15q), del(17)(p11)x1-2, add(22)(p11)x2 - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
