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Cell line: | TC-71 |
DSMZ no.: | ACC 516 |
Species: | human (Homo sapiens) |
Cell type: | Ewing's sarcoma |
Origin: | established from the tumor of a 22-year-old man with metastatic Ewing's sarcoma that arose in the humerus; cell line was derived in 1981 from a biopsy of recurrent tumor at the primary site |
Reference(s): | 14948, 15058 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | loosely adherent cells growing in monolayers; image |
Medium: | 90% Iscove's MDM + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:10 every 2-4 days; trypsin/EDTA is not necessary, cells can be detached by tapping the flask |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 24 hours |
Harvest: | cell harvest of about 15 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypertriploid karyotype with 4% polyploidy - 70-80<3n>XX, -Y, +1, +2, -3, +6, +8, -9, +11, -17, +21, +2-11 mar, add(3)(q29), add(6)(q25)x2-3, del(8)(p22), add(8)(p22), del(9(p24), t(11;22)(q24;q12)x2, i(13q)x1-2, add(14)(p11) - sideline with der(2)t(2;3)(p23;p11), add(11)(p15)x2 - carries two copies of t(11;22) effecting EWS-FLI-1 fusion, yielding type 1 (shorter) product - broadly resembles published karyotype |
Molec. Genetics: | expression of fusion gene EWS-FLI (type 1) confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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