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| Cell line: | TALL-1 |
| DSMZ no.: | ACC 521 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia |
| Origin: | established from the bone marrow of a 28-year-old man who developed the terminal leukemic phase of lymphosarcoma in 1976 |
| Reference(s): | 14772 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single cells growing in suspension; image; image; image |
| Medium: | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 3-4 days; maximal density of ca. 2.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-70 hours |
| Harvest: | cell harvest of about 2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays |
| Immunology: | CD2 +, cyCD3 +, CD4 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertetraploid karyotype - 90-102<4n>XXY/XXYY, -3, -9, -12, +13, +14, +14, -15, +20, +20, +21, +21, +2-4mar, der(X;1)(p10;p10)/add(1)(q11), add(1)(p11), add(5)(p1?5), del(2)(p22p24), del(12)(q24.1), dup(21)(q11qter)x3-4 - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
