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Cell line: | DND-41 |
DSMZ no.: | ACC 525 |
Species: | human (Homo sapiens) |
Cell type: | T cell leukemia |
Origin: | established from the peripheral blood of 13-year-old boy with T-acute lymphoblastic leukemia (T-ALL; type III cortical) in 1977; carries an activating insertional mutation in the IL7R; described in the literature to carry CDKN2B (p15INK4B) and CDKN2A (p16INK4A) deletions and TP53 (p53) mutation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 14473, 15217, 18187, 40915 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly or, occasionally, in clumps; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; maintain at ca. 0.3-1.0 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35 hours |
Harvest: | cell harvest of ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, RNA hybridization, and continuously PCR assays |
Immunology: | CD3 +, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded near-tetraploid karyotype with 12% polyploidy - 92(86-98)<4n>XXYY, -9, -15, +20, +mar - resembles published karyotype |
Molec. Genetics: | expression of TLX3 confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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