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Cell line: | NTERA-2 |
DSMZ no.: | ACC 527 |
Species: | human (Homo sapiens) |
Cell type: | embryonal carcinoma (teratocarcinoma) |
Origin: | NTERA-2 was cloned from cell line TERA-2 which was derived from a metastatic teratocarcinoma of a 22-year-old male; cell line also known as NT-2 |
Reference(s): | 14358, 15130, 15135 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epitheloid cells carrying strong granula growing adherent in monolayer |
Medium: | 85% DMEM + 10% h.i. FBS + 5% horse serum |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 about 1-2 times a week using trypsin/EDTA; after thawing use collagen-coated flasks for the first week |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50-60 hours |
Harvest: | cell harvest of about 14 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hyperdiploid karyotype - 48-58<2n>XXXY, +1, +16, +20, +22, +3-4mar, add(X)(p11), add(X)(q2?5), der(X;1)(p10;q10), add(1)(p35-36), add(11)(p15), add(13)(q3?1) - sideline with i(3q) |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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