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| Cell line: | OCI-M1 |
| DSMZ no.: | ACC 529 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established from erythroleukemia blasts (AML M6) of a 62-year-old patient following a 7-year chlorambucil treatment for chronic lymphocytic leukemia (CLL); described in the literature to express many markers of the erythroid lineage as well as several non-erythroid markers |
| Reference(s): | 15026, 14834, 14833 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; image; image; image; image |
| Medium: | 80-90% Iscove's MDM + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-0.3 x 106 cells/ml; split saturated culture 1:2 to 1:4 every second day; maximal density of ca. 1 x 106 cells/ml; immediately after thawing viability may be between 70 and 80% |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD4 -, CD15 +, CD19 -, CD33 +, CD34 -, CD41 -, CD42 -, CD71 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
