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Cell line: | JURL-MK1 |
DSMZ no.: | ACC 532 |
Species: | human (Homo sapiens) |
Cell type: | chronic myeloid leukemia in blast crisis |
Origin: | established from the peripheral blood of a 73-year-old man with chronic myeloid leukemia (CML) in blast crisis in 1993; cells carry the Philadelphia chromosome; JURL-MK1 and JURL-MK2 are sister cell lines derived from the same patient |
Reference(s): | 14459 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly or in small clusters in suspension; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | cell harvest of ca. 2.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD4 -, CD14 -, CD15 +, CD19 -, CD33 +, CD41 +, CD71 +, CD117 +, HLA-DR + |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypodiploid karyotype with 4% polyploidy - 45(41-45)<2n>XY, -9, add(1)(p35), der(9)del(9)(p21.2)t(9;22)(q34;q11), der(11)t(11;9;22)(p15;q34;q11), add(12)(p13)x2, add(17)(p13), der(22)t(9;22)(q34;q11) - carries t(9;22) effecting BCR-ABL1 fusion (two copies) - does not appear to match published karyotype |
Molec. Genetics: | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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