| | |
|---|
| Cell line: | HCC-44 |
| DSMZ no.: | ACC 534 |
| Species: | human (Homo sapiens) |
| Cell type: | non-small cell lung carcinoma |
| Origin: | established from the lung of a 54-year-old woman with non-small cell lung cancer of the adenocarcinoma type; listed in the CCLE (ref 18232) with extended genome and protein analyses |
| Reference(s): | 15046, 14821, 14849, 18232, 40404 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epitheloid cells growing as monolayer; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of about 4-6 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with sparfloxacin, thereafter negative in microbiological culture and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hyperpentaploid karyotype with 8% polyploidy - 120-124<5n>XX, +1, +1, +3, +3, -4, -4, -5, +7, +7, +7, -9, -9, -10, +11, -18, -21, -21, +22, +22, +22, +7-14mar, der(1)t(X;1)(q11;p11)x2, del(1)(q21)x2, i(1q), t(1;3)(p11;q11), add(2)(q?11), del(2)(p11), add(3)(q28), add(3)(p11), i(5p), add(8)(p2?), add(8)(q2?), add(8)(q24), der(9;19)(q10;q10)x2, add(10)(q2?5), add(11)(p13), der(11)add(11)(p1?1)add(11)(q25), add(12)(p13), add(16)(p11), add(17)(p1?2), add(18)(p11), del(20)(q12)x1-2, add(20)(q13)x2 - 2-6 dmin present in 80% cells |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
