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| Cell line: | DB |
| DSMZ no.: | ACC 539 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from ascites of a 45-year-old man with diffuse large cell lymphoma; cell line carries EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s): | 14375, 16553, 17354 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round single cells of different sizes, growing in suspension and partly in clusters; image; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; minimum density at ca. 0.4 x 106 cells/ml; after thawing cultivate in 6-well-plates during the first 2 weeks |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | cell harvest of ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4-, CD10+, CD13-, CD19(+), CD20+,
CD34-, CD37+, CD38(+), CD80+, CD138(+),
HLA-DR-, sIgG+, sIgM-, sIg-kappa+, sIg-lambda+,
cIgG+, cIgM-, cIg-kappa+, cIg-lambda+, cyCD79a+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypertriploid karyotype - 80(79-81)<3n>XXXYY, -3, +7, +7, +8, -9, +11, -13, +16, +18, +20, +20, +20, +21, +22, +mar, der(4)t(X;4)(q11;p13), add(5)(q11), t(10;20)(q24;p11), ?inv(13)(q21.2q32), t(14;18)(q32;q21), del(16)(p13.2), der(18)(t(14;18)(q32;q21) - carries t(14;18) associated with B-cell lymphoma |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
