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Cell line: | CAKI-2 |
DSMZ no.: | ACC 54 |
Species: | human (Homo sapiens) |
Cell type: | kidney carcinoma |
Origin: | established from the primary renal carcinoma of a 69-year-old man |
Reference(s): | 14537, 14538 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like cells, growing as adherent monolayer; image; image; image |
Medium: | 90% McCoy's 5A + 10% h.i. FBS |
Subculture: | change medium twice every week, split confluent cultures 1:2 to 1:3 weekly using trypsin/EDTA, upon thawing grow cells immediately in 80 cm2; seed out at ca. 2 x 106 cells/80 cm2 in 10 ml medium |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 1 x 106 cells/25 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded pentaploid/hexaploid karyotype with 12% polyploidy - 130(118-132)<6n>XXX, -Y, -Y, -Y, inc, add(1)(p22), i(8q), i(?5q)x2, del(6)(q23), t(14;15)(p10;q10), der(19)t(1;19)(q24;q13), dminx1, large iso der(5q)x2, and submeta marker der(?)t(?;2)(?;q11)x2 - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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