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Cell line: | H-1963 |
DSMZ no.: | ACC 540 |
Species: | human (Homo sapiens) |
Cell type: | small cell lung carcinoma |
Origin: | derived from the lung of a 56-year-old man with small cell lung carcinoma prior to treatment in 1988 (corresponds to NCI-H1963); listed in the CCLE (ref 18232) with genome and protein analyses |
Reference(s): | 14849, 14567, 18232, 40405 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | cells grow in clumps in suspension; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | split culture 1:2 every 3 days; seed out at ca. 0.5 x 106 cells/ml; cell number is difficult to determine due to the formation of large aggregates |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 3-4 days |
Harvest: | cell harvest of ca. 1-2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human bimodal hypodiploid/hypotetraploid aryotype with 3% polyploidy - 44(42-48)<2n>X, -Y, -5, -10, -13, +18, -19, +2mar, add(1)(q32), ins(2;hsr)(p21-23;?), add(3)(p11), del(4)(q24-26), del(10)(p13-14), add(18)(p11)x2, del(20)(q12), der(22)(t1;22)(q12;q13), dmin present in some cells |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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