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| Cell line: | SEM |
| DSMZ no.: | ACC 546 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from the peripheral blood of a 5-year-old girl at relapse of acute lymphoblastic leukemia (ALL) in 1990; reported to carry the t(4;11) KMT2A-AFF1 (MLL-AFF1; MLL-AF4). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
| Reference(s): | 14577, 14493, 14887, 40437, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polygonal single rather small cells in suspension; image; image; image |
| Medium: | 90% Iscove's MDM + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:6 every 2-3 days; maximum density at ca. 3-4 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 3-4 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10-, CD13+, CD19+, CD20-, CD34-, CD37-, CD38-, CD80-, CD138-, HLA-DR+, cyCD79a+, sIgG-, cIgG-, sIgM-, cIgM-, sIg-kappa-, cIg-kappa-, sIg-lambda-, cIg-lambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 1.5% polyploidy - 45(40-46)<2n>XX, -13, t(4;11)(q21;q23), del(7)(p14) - carries t(4;11) with breakpoints at AFF1 (AF4) and KMT2A (MLL) - matches published karyotype |
| Molec. Genetics: | expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
