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| Cell line: | AML-193 |
| DSMZ no.: | ACC 549 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established from a 13-year-old girl with acute myeloid leukemia (AML FAB M5) at relapse |
| Reference(s): | 14703, 14482 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorph cells growing singly or in small clusters in suspension; image |
| Medium: | 70% Iscove's MDM + 10% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSMZ ACC 35) |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4+, CD13-, CD14-, CD15+, CD16-, CD19-, CD33+, CD34-, HLA-DR-, cyCD3-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 49(46-52)<2n>XX, +3, +6, +8, +13, i(17q) - matches published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
