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Cell line: | A-498 |
DSMZ no.: | ACC 55 |
Species: | human (Homo sapiens) |
Cell type: | kidney carcinoma |
Origin: | established from the kidney carcinoma of a 52-year-old man in 1973 |
Reference(s): | 14556, 18168 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like, adherent cells growing as monolayers; image; image; image; image; image |
Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS |
Subculture: | seed out at ca. 1.0 x 106 cells/80 cm2; split confluent cultures 1:3 to 1:5 every 3-5 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60-70 hours |
Harvest: | about 2-3 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype with 7% polyploidy - 72(68-72)<3n>XX, -X, +2, -3, -6, +7, +7, +9, -11, -14, +17, -18, -20, +21, +3mar, dm/r (31%) - der(1)t(1;1)(p32;q12), der(2)t(2;3)(q37;q13), i(8q) - sideline with der(3)t(3;3)(q27;p14) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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