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Cell line: | MOLM-13 |
DSMZ no.: | ACC 554 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid leukemia |
Origin: | established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndrome (MDS, refractory anemia with excess of blasts, RAEB); described to carry internal tandem duplication of FLT3; the FLT3 protein is not expressed; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-14 (ACC 777). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 14869, 14743, 14493, 15073, 16558, 18138, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | most cells are round growing in suspension; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca 0.4-2.0 x 106 cells/ml; viability may drop below 50% after thawing, but cells recover after 4-5 days (starting in a 12- or 24-well plate is recommended) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of ca. 2.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD4 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cy CD68 +, HLA-DR -
; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MOLM-14 |
Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 51(48-52)<2n>XY, +8, +8, +8, +13, del(8)(p1?p2?), ins(11;9)(q23;p22p23) - resembles published karyotype - sideline with idem, +19 - carries occult insertion effecting KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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