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Cell line: | SC-1 |
DSMZ no.: | ACC 558 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established from the ascitic fluid of a 67-year-old man at diagnosis of B cell lymphoma (B-NHL, follicular lymphoma, small cleaved cell type) in 1977 |
Reference(s): | 14951, 15013, 40740 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | lymphoid cells growing singly or in small clumps in suspension; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 1.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 3-5 days; maintain at 1-2 x 106 cells/ml; max. density at ca. 3.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30 hours |
Harvest: | cell harvest of ca. 3 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, cyCD79a (+), CD80 +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cy lambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 47(43-48)<2n>XY, +7, -17, +mar, add(3)(q27), t(8;14;18)(q24;q32;q21), add(14)(q32) - carries 3-way t(8,14,18) effecting genomic co-amplification of BCL2 and MYC - resembles published karyotype |
Molec. Genetics: | expression of fusion gene BCL2-IGH confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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