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Cell line: | HCC-78 |
DSMZ no.: | ACC 563 |
Species: | human (Homo sapiens) |
Cell type: | non-small cell lung carcinoma |
Origin: | established from the pleural effusion of a 65-year-old man with adenocarcinoma of the lung, typed as non-small cell lung carcinoma; cells are described in the literature to carry a ROS1 rearrangement; listed in the CCLE (ref 18232) |
Reference(s): | 15046, 14567, 19109, 14849, 40398, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | large epitheloid cells growing in monolayers and aggregates; after trypsination cells are not always dispersed as single cells; image; image
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Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out 1-2 x 106 cells in 175 cm2; split confluent culture 1:2 twice a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 60-70 hours |
Harvest: | cell harvest of ca. 8-10 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | originally negative in microbiological culture, and later continuously by PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded complex hypotetraploid karyotype with 6% polyploidy - 80-91<4n>XYY/XXYY, add(X)(q25)x2, add(1)(?p11), add(4)(p15), add(4)(q31), add(16)(q23), der(16)t(1;16)(p12;q23)x2, dup(16)(q11q13)add(16)(q13), add(19)(q13)x2, inc. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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