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Cell line: | EN |
DSMZ no.: | ACC 564 |
Species: | human (Homo sapiens) |
Cell type: | endometrial carcinoma |
Origin: | established from the primary endometrial (cervical) carcinoma (stage IIIC) which was histologicall poorly differentiated from a 50-year-old woman prior to therapy |
Reference(s): | 14630 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | adherent epitheloid cells growing in monolayers; image; image; image |
Medium: | 80% DMEM + 20% h.i. FBS |
Subculture: | split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out 1.5 x 106 cells in 80 cm2; maintain at 0.1-0.2 x 105 cells/cm2; after recovery from N2 cell growth is slow (doubling time > 120 h) |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50-80 hours |
Harvest: | cell harvest of ca. 1 x 105 cells/cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded near-tetraploid karyotype with 3% polyploidy - 79-97<4n>XXX/XXXX, -13, +2mar, 0-4 dm, der(1;7)(q10;q10)x2 - resembles published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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