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Cell line: | HCC-827 |
DSMZ no.: | ACC 566 |
Species: | human (Homo sapiens) |
Cell type: | non-small cell lung carcinoma |
Origin: | established from the lung of a 39-year-old woman with non-small cell lung cancer of the adenocarcinoma type; extensively characterized cell line (see ref 40398 and Cellosaurus:Expasy; listed in the CCLE (see ref 18232) |
Reference(s): | 14567, 15046, 18232, 40404, 40398 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | large epitheloid cells growing in monolayers; image; image; image; image; image |
Medium: | 80-85% RPMI 1640 + 15-20% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split saturated culture 1:2 to 1:3 about twice a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50-60 hours |
Harvest: | cell harvest of ca. 5-6 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypotriploid karyotype with 6% polyploidy - 62(54-66)<3n>XXX/XXXX, -1, +7, +7, -9, -10, -15, -17, -18, -21, -22, +3 mar, +0-4 dmin, add(X)(p11), dup(2)(p1?p2?), add(3)(p11), add(6)(p21), add(7)(p12), add(7)(p22), add(7)(q21.2;HSR), add(21)(p11), der(21;22)(q10;q10), add(22)(p11) - 2 large submetacentric HSR markers present |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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