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| Cell line: | HT |
| DSMZ no.: | ACC 567 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the ascitic effusion of a 70-year-old man with B cell non-Hodgkin lymphoma (B-NHL) in 1983 (diffuse large cell lymphoma); assigned to GCB-like lymphoma subtype (germinal center B-cell) |
| Reference(s): | 14375, 17355 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small round cells growing as clumps in suspension; image; image |
| Medium: | 85-90% RPMI 1640 + 10-15% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 3-4 days; maintain at 0.5-1 x 106 cells/ml; max. density at about 1.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | cell harvest of ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 3% polyploidy - 46(42-46)<2n>XY, +2, der(2;4)(p10;q10), der(2)(del)(2)(p11?q21), dup(10)(q11q22-23), dup(11)(?q23qter) - no IGH-BCL2 rearrangement detected |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
