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| Cell line: | SU-DHL-8 |
| DSMZ no.: | ACC 573 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the pleural effusion of a 59-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse large cell lymphoma, noncleaved cell type and later as diffuse histiocytic lymphoma; assigned to GCB-like lymphoma subtype (germinal center B-cell); carries the t(8;22) translocation affecting c-myc |
| Reference(s): | 14515, 15065, 14605, 17354, 14516 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round single cells growing in suspension; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 2-3 days; maintain at ca. 1-2 x 106 cells/ml; maximal density at 3-4 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48-72 hours |
| Harvest: | cell harvest of ca. 3-4 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10-, CD13-, CD19+, CD20+, CD34-, CD37+, CD38+, CD80+, CD138-, HLA-DR+, sIgG+, sIgM-, sIg-kappa+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 3% polyploidy - 51(47-53)<2n>X, -Y, +7, +13, +20, +20, t(8;22)(q24;q11), del(20)(q13), der(22)t(1;22)(q11;p11)del(1)(q?32) - carries t(8;22) effecting IGL-MYC rearrangement - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
