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Cell line: | SU-DHL-16 |
DSMZ no.: | ACC 577 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established from the pleural effusion of a man with B-cell non-Hodgkin lymphoma (B-NHL) |
Reference(s): | 15039, 15062 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | round to polymorph cells growing in clumps; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml in a 24-well-plate, after thawing viability may be less than 70% but the cells recover during the following 3-4 days; maintain at ca. 0.3-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:6 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40 hours |
Harvest: | cell harvest of ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 +, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD138 -, HLA-DR -, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hyperdiploid karyotype with 3% polyploidy - 47(44-49)<2n>XXY, +10, -13, +15, t(1;5)(q31;q21), add(2)(q21-22), der(2)t(2;2)(q3?7;q21-22)t(2;10)(p25;q24), der(10)t(2;10)(p25;q24)x2, del(12)(p12)dup(12)(q11q22), der(14)t(13;14)(q11;p11)t(14;18)(q32;q21), add(15)(q23)x2, i(17q), der(18)t(14;18)(q32;q21) - carries t(14;18) effecting BCL2-IGH rearrangement |
Molec. Genetics: | expression of fusion gene BCL2-IGH confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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