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Cell line: | HTC-C3 |
DSMZ no.: | ACC 580 |
Species: | human (Homo sapiens) |
Cell type: | thyroid carcinoma |
Origin: | established from cancer cells disseminated in the pleural fluid of a 44-year-old woman with undifferentiated giant cell carcinoma of the thyroid after chemotherapy, x-ray hyperthermia and OK432 injections in 1986; cells were described in the literature to contain parathyroid-like activity |
Reference(s): | 14512 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like cells growing as monolayer; image; image; image |
Medium: | 80% DMEM (4.5 g/L glucose) + 20% h.i. FBS |
Subculture: | seed out 0.2-0.5 x 106 cells in 80 cm2; split confluent culture 1:2 once a week using trypsin-like enzyme (e.g. Tryple Express) (standard trypsin/EDTA is not sufficiently effective); note that viability may decrease rapidly after reaching confluency |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 100 hours |
Harvest: | cell harvest of ca. 12 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-triploid karyotype with 23% polyploidy - 68(68-73)<3n>XXXXX, add(X)(p11), add(X)(p11)add(X)(q11), add(X)(q11), del(X)(p11), der(6)t(X;6)(q23;q25), inc. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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