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| Cell line: | 7926 |
| DSMZ no.: | ACC 587 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | acute myeloid leukemia |
| Origin: | murine radiation-induced acute myeloid leukemia (AML) cell line derived from splenocytes of the C3H inbred mouse strain in 1997; cell line was described in the literature to be radio-sensitive |
| Reference(s): | 14534, 15204, 15205 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | mostly round cells growing in clumps or singly in suspesion |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + (10 ng/ml mIL-3) |
| Subculture: | split saturated culture 1:2 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; cells are very difficult to culture, mIL-3 supports growth of the cells, cells grow best in 12- or 24-well-plates; after thawing, for the first 7-10 days there is virtually no cell growth or cells grow very slowly, viability may be initially very low, not exceeding 80-85% |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-80 hours |
| Harvest: | maximum density at about 1-1.2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
