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Cell line: | NIH-3T3 |
DSMZ no.: | ACC 59 |
Species: | murine (mouse, Swiss mouse) (Mus musculus) |
Cell type: | embryo fibroblasts |
Origin: | NIH Swiss mouse embryo; used in DNA transfection assays; described as contact inhibited |
Reference(s): | 14343, 14440, 14637 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | fibroblastoid, adherent cells growing as monolayer; image; image; image; image
image; image |
Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS (or NCS) |
Subculture: | seed out at 1-2 x 106 cells/175 cm2 flask;
do not allow culture to become completely confluent; split culture up to 1:10 every 4 to 6 days using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 20 hours |
Harvest: | cell harvest of about 20 x 106 cells/175 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine hypertriploid karyotype with 3% polyploidy - 68(58-73)<3n> |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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