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Cell line: | JIYOYE |
DSMZ no.: | ACC 590 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | cell line JIYOYE (also known as JIJOYE, P-2003 or P-3) was established from a 7-year-boy with Burkitt lymphoma in 1965 |
Reference(s): | 14683, 14863 |
Biosafety level: | 2 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
Permissions and restrictions: | A, B |
| DSMZ Cell Culture Data: |
Morphology: | lymphoid cells growing in suspension, singly or in clumps; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | split saturated culture 1:2 to 1:4 every 2-4 days; seed out at ca. 0.5-0.8 x 106 cells/ml in 24-well-plates in 20% FBS; after recovery, viability may be low! maintain at ca. 0.3-1.0 x 106 cells/ml; maximum density at ca. 2.0 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-60 hours |
Harvest: | maximum density at about 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +,
CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded near-diploid karyotype with 13% polyploidy - 42-48<2n>XY, der(7)t(1;7)(q32;q32), t(8;14)(q24;q32), i(9p), der(14)t(9;14)(q11;q32), +0-2mar; carries t(8;14) rearranging IGH with MYC (far upstream breakpoint) |
Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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