| | |
|---|
| Cell line: | MOLM-20 |
| DSMZ no.: | ACC 591 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic neutrophilic leukemia |
| Origin: | established from the peripheral blood of a 63-year-old woman with chronic neutrophilic leukemia (CNL) in transformation/blast crisis in 2003; MOLM-20 was established independently in another laboratory and is a sister cell line of CNLBC1 (see references). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14684, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round cells growing in suspension; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | cells are difficult to culture; seed out at ca. 2 x 106 cells/ml in a 24-well-plate; maintain at ca. 1-2 x 106 cells/ml in a 24-well-plate; split saturated culture 1:2 once or twice per week; post thawing cells may need 5-7 days to recover and to reach viabilities above 80% |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70 hours |
| Harvest: | maximum density at about 4 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD34 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 8% polyploidy; 49(47-50)<2n>XXX, +X, +8, +21, t(4;11)(q21.1;q23); carries MLL rearrangement with 4q21.1 breakpoint outside (centromeric) of AFF1 (AF4); matches karyotype of sister cell line CLNBC1 |
| Molec. Genetics: | expression of fusion gene KMT2A-SEPT11 (MLL-SEPT11; MLL-FLJ10849) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
